Eggcellent Macromolecules Lab
In this lab, we asked "can macromolecules be identified in an egg cell?" We found that we can identify macromolecules in an egg with the right solution to test it. For the egg membrane, we found lipids present from the color change, after submerging the egg into the Sudan III and water solution. The solution, originally bright red, changed to a medium red-ish orange. Lipids make up the membrane and stores energy. The egg white contained traces of protein when it showed the color change from blue to a dark purple, due to the added sodium hydroxide and copper sulfate. The cell produces the structural protein to feed the growing chick inside the egg. It also serves as protection. Lastly, there was polysaccharides in the egg yolk. With the help of another group's data, we concluded that the chestnut brown solution, iodine, changed to a blackish color. It stores energy for the cell, or the developing chick.
This lab had many issues and errors to it. For one, our monosaccharide test had different results that we were looking for. Looking for monosaccharides in the egg membrane and white, we found a different macromolecule present. The solution turned that part of the egg to a different color, but not the specific color of green or orange. Instead, the solution changed the egg to purple. I think that the different color change was accidental and would not occur if we were to do this lab again. In the lipid test, our data contradicts the expected results because the yellow yolk didn't mix with the solution. That could have affected the color during the color observation portion. In future experiments, I would recommend mixing the solution and the part of the egg completely to get the correct observation.
This lab was done to demonstrate if macromolecules can be identified in an egg cell. From this lab, I understand the structure of the cell due to where the macromolecule is located within it and the importance of each of them in one single cell. For example, the protein is located in the membranes, cytosplasm, organelles, and cytoskeleton because it helps provide energy for the unborn chick. I can apply this lab to outside situation such as testing another cell like object for macromolecules. In the photos below is the before and after the solution was contaminating the egg during the monosaccharide test.
This lab had many issues and errors to it. For one, our monosaccharide test had different results that we were looking for. Looking for monosaccharides in the egg membrane and white, we found a different macromolecule present. The solution turned that part of the egg to a different color, but not the specific color of green or orange. Instead, the solution changed the egg to purple. I think that the different color change was accidental and would not occur if we were to do this lab again. In the lipid test, our data contradicts the expected results because the yellow yolk didn't mix with the solution. That could have affected the color during the color observation portion. In future experiments, I would recommend mixing the solution and the part of the egg completely to get the correct observation.
This lab was done to demonstrate if macromolecules can be identified in an egg cell. From this lab, I understand the structure of the cell due to where the macromolecule is located within it and the importance of each of them in one single cell. For example, the protein is located in the membranes, cytosplasm, organelles, and cytoskeleton because it helps provide energy for the unborn chick. I can apply this lab to outside situation such as testing another cell like object for macromolecules. In the photos below is the before and after the solution was contaminating the egg during the monosaccharide test.
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