pGLO Lab

pGLO Observations , Data Recording & Analysis
1.
Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below.
Plate
Number of Colonies
Color of colonies under room light
Color of colonies under UV light
- pGLO LB
8
Tan-ish/white, opaque
Light purple
- pGLO LB/amp
0
No bacteria
No bacteria
+ pGLO LB/amp
1
Tan-ish/white,
opaque
Light purple
+ pGLO LB/amp/ara
4
Tan-ish/white, opaque
Neon green

2.
What two new traits do your transformed bacteria have?
The transformed bacteria, which is the dish that contain LB/amp/ara, glows underneath the UV light. This is an effect of plasmid that turns the bacteria into a green fluorescent color. The plate also carries agarose sugar, the only thing that can make the bacteria glow if the plasmid is not present. The bacteria was resistant to ampicillin, which would potentially kill it.
3.
Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
I believe there are more than a thousand bacteria on that dish. You can’t see these tiny cells with the human eye, but when it’s enhance under a microscope, you can see that it is multiplying every single second.
4.
What is the role of arabinose in the plates?
Arabinose is like a switch that turns on the gene for GFP. It is an indication that you have bacteria in the plasmid.
5.
List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
  1. Living laser: putting an eGFP-bearing cell inside a reflective optical hole and hitting it with pulsing blue light
  2. GFP infused rabbit: engineering GFP into the cells of the rabbit and now other living things
  3. Tagging marine bacteria: adding GFP to locate where the expression in the cell is
6.
Give an example of another application of genetic engineering.
In agriculture, genetic engineering is used to delay ripening for transportation purposes and become resistant to insects

Under room light 

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