In this disgusting lab, we asked the question, "How can DNA be separated from cheek cells in order to study it". We concluded that if we wanted to separate DNA from our rosy cheeks, we'd have to do a number of steps. Three steps, to be exact. Those steps would be homogenization , lysis , and precipitation . To achieve homogenization, we used Gatorade to break down the cell tissue. Because of Gatorade's polarity , we could use it. Gatorade has electrolytes, like sodium and potassium, that athletes sweat out during physical activity. We took a shot of Gatorade and swished it in out mouths, hoping to gain the cheek cells needed. After, we spitted it back into a cup. The next step was lysis. We added salt and detergent into the mix, to emulsify the proteins and lipids of the cell. Next, was the protease solution: pineapple juice (tempted to make a pen-pineapple-apple-pen joke). The pineapple juice is a catabolic protease, which is to break down the histones further. M...
Comments
Post a Comment